In vivo induced antigenic determinants of group A Streptococcus
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In vivo induced antigenic determinants of group A Streptococcus

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Published in 2007 .
Written in English


Book details:

Edition Notes

Thesis (Ph.D.)--University of Toronto, 2007.

Statementby Kowthar Yislam Salim.
The Physical Object
Paginationxix, 212 leaves :
Number of Pages212
ID Numbers
Open LibraryOL19552316M

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Identification of Group A Streptococcus Antigenic Determinants Upregulated In Vivo Kowthar Y. Salim, 1Dennis G. Cvitkovitch, Peter Chang,2 Darrin J. Bast,2 Martin Handfield,3 Jeffrey D. Hillman,3,4 and Joyce C. S. de Azavedo2* Department of Microbiology, Faculty of Dentistry,1 and Faculty of Laboratory Medicine and Pathobiology,2Cited by:   Identification of group A Streptococcus antigenic determinants upregulated in vivo. (PMID PMCID:PMC) We identified genes expressed during invasive disease using in vivo-induced antigen technology (IVIAT), applied for the first time in a gram-positive organism. seven genes were assessed for their differential expression. Identification of Group A Streptococcus Antigenic Determinants Upregulated In Vivo. By Kowthar Y. Salim, Dennis G. Cvitkovitch, Peter Chang, Darrin J. Bast, Martin Handfield, Jeffrey D. Hillman and Joyce C. S. de Azavedo. Abstract. Group A Streptococcus (GAS) causes a range of diseases in humans, from mild noninvasive infections to severe. By screening for in vivo-induced antigens, Identification of group A streptococcus antigenic determinants upregulated in vivo. Infect. Immun. – [PMC free article] Sivick K. E., Mobley H. L. An “omics” approach to uropathogenic Escherichia coli vaccinology.

Six in vivo-induced (IVI) antigens—RnhB, GalU, GalT, Apl_, Apl_, and HflX were selected for a vaccine trial in a mouse results showed that the IgG levels in each immune group was significantly higher than that of the negative control (P group, proliferation of splenocytes was observed in all immunized groups and a relatively higher proliferation. SUMMARY Group A streptococci are model extracellular gram-positive pathogens responsible for pharyngitis, impetigo, rheumatic fever, and acute glomerulonephritis. A resurgence of invasive streptococcal diseases and rheumatic fever has appeared in outbreaks over the past 10 years, with a predominant M1 serotype as well as others identified with the outbreaks. emm (M protein) gene .   Streptococcus pneumoniae (a major cause of human pneumonia) and Streptococcus mutans and other so-called viridans streptococci (among the causes of dental caries) do not possess group antigens. Three types of hemolysis reaction (alpha, beta, gamma) are seen after growth of streptococci on sheep blood agar.   Infection with Streptococcus pyogenes, a beta-hemolytic bacterium that belongs to Lancefield serogroup A, also known as the group A streptococci (GAS), causes a wide variety of diseases in humans. A ubiquitous organism, S pyogenes is the most common bacterial cause of acute pharyngitis, accounting for % of cases in children and % of.

  Adsorption of antisera. To identify antigens that were expressed only in vivo, it was necessary to remove antibodies induced by the antigens expressed in tion of primary antisera was done using a method described previously (Handfield et al., ).The relevant stages were essentially as follows: (i) pooled primary antisera (sera 0) were mixed twice with whole cells of SE. Group A streptococcus (GAS) is commonly associated with acute pharyngitis and impetigo, as well as a wide variety of severe invasive diseases [].Infection begins with colonization of the upper respiratory tract or injured skin surfaces, a critical step in pathogenesis [].Although not considered normal flora, asymptomatic GAS colonization varies between 5% and 20% in healthy individuals [3– 6. M.H.V. Van Regenmortel, in Encyclopedia of Virology (Third Edition), Antigenicity or antigenic reactivity refers to the capacity of viruses to bind to specific antibody molecules. The antigenicity of nonenveloped viruses resides in the antigenic sites or B-cell epitopes of capsid proteins that are recognized by the binding sites of antibodies.   Abstract. Porphyromonas gingivalis is a Gram-negative anaerobic bacterium associated with the initiation and progression of adult periodontal disease. The pathogenicity of P. gingivalis is multifaceted and the infection process is influenced by both microbial and host factors. It is generally accepted that genes of a pathogen that are specifically expressed during infection are likely to be.